Analysis of the solubilized nicotinic acetylcholine receptor ofDrosophila melanogaster
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منابع مشابه
Binding of perhydrohistrionicotoxin to intact and detergent-solubilized membranes enriched in nicotinic acetylcholine receptor.
A tritiated derivative of perhydrohistrionicotoxin was prepared and characterized and used in direct binding studies to membrane fragments from Torpedo californica electroplax. Perhydrohistrionicotoxin bound to a site distinct from the a-bungarotoxin and agonist binding sites in a ratio of one perhydrohistrionicotoxin bound per four a-bungarotoxin sites. In the presence of 10 pM carbamylcholine...
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We report here the biologically active conformation of acetylcholine when bound to the high-affinity state of the receptor from Torpedo californica. The acetylcholine conformation was determined in the free and bound states by proton NMR two-dimensional nuclear Overhauser effects. In agreement with x-ray crystallographic data, acetylcholine in solution has an extended conformation with an avera...
متن کاملEmbedded cholesterol in the nicotinic acetylcholine receptor.
The nicotinic acetylcholine receptor (nAChR) is a cation-selective channel central to both neuronal and muscular processes and is considered the prototype for ligand-gated ion channels, motivating a structural determination effort that spanned several decades [Unwin N (2005) Refined structure of the nicotinic acetylcholine receptor at 4 A resolution. J Mol Biol 346:967-989]. Purified nAChR must...
متن کاملMutational analysis of muscle nicotinic acetylcholine receptor subunit assembly
The structural elements required for normal maturation and assembly of the nicotinic acetylcholine receptor alpha subunit were investigated by expression of mutated subunits in transfected fibroblasts. Normally, the wild-type alpha subunit acquires high affinity alpha bungarotoxin binding in a time-dependent manner; however, mutation of the 128 and/or 142 cysteines to either serine or alanine, ...
متن کاملDiversity of insect nicotinic acetylcholine receptor subunits.
Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that mediate fast synaptic transmission in the insect nervous system and are targets of a major group of insecticides, the neonicotinoids. They consist of five subunits arranged around a central ion channeL Since the subunit composition determines the functional and pharmacological properties of the receptor the presence o...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1980
ISSN: 0014-5793
DOI: 10.1016/0014-5793(80)80587-4